Regenerated cellulosic fibers, such as lyocell fibers, are fully biodegradable and of 100% natural origin. With properties like high dry tenacity, they are widely used in many high-quality fiber applications. Among cellulosic fibers, lyocell fibers exhibit the highest dyeability, particularly with synthetic reactive dyes. However, this results in high dye yields and significant amounts of reactive dye being lost in the effluent during the wash-off process, leading to energy-intensive water purification and environmental contamination. Cationic dyes, which have a high affinity for substrates and better dye uptake, can reduce dye wastage. To enhance the affinity of lyocell fabric for cationic dyes, the fabric surface must be modified to introduce a negative charge. An elegant solution involves the use of the enzyme lytic polysaccharide monooxygenase (LPMO), which specifically hydroxylates the C1 carbon position of the glycosidic bond without degrading the fabric. This research demonstrates that dye retention can be increased by up to 75% for rhodamine-6G dye with LPMO loadings of ≤ 1%. Additionally, treated fabrics outperformed non-treated fabrics, achieving similar dye uptake with half the amount of dye used.