Global fiber production has exceeded 110 million tons annually, with approximately 72% consisting of man-made fibers derived from natural or synthetic polymers. While these fibers are essential to meet market demands, their heavy reliance on fossil-based raw materials poses significant ecological, economic, and political challenges. Consequently, there is a growing demand for sustainable alternatives, including biomass and post-consumer waste. A less explored yet promising source is discarded human hair, which is estimated to be 150 Ktonnes annually in Europe and 2000 Ktonnes globally that goes to incinerators. Rich in keratin—a fibrous protein with high potential for fiber spinning—human hair remains underutilized in textile applications. The primary challenge in using keratin lies in its non-linear polymer structure, which complicates spinning and results in fibers with lower mechanical properties. To address this, AMIBM and Human Material Loop conducted spinning trials using keratin extracted from human hair. Various methods, including blending keratin with biopolymers such as cellulose and solution spinning of pure keratin, were explored to assess its potential for textile applications. The impact of factors like solvent type, polymer blends, and draw ratios on fiber properties have been taken into consideration during the trials. This study dives into the potential of keratin as a viable textile fiber, highlighting an innovative approach to upcycle waste material into value-added products.